Journal: BMC Cancer

Article Title: LMOD1, an oncogene associated with Lauren classification, regulates the metastasis of gastric cancer cells through the FAK-AKT/mTOR pathway

doi: 10.1186/s12885-022-09541-0

Figure Lengend Snippet: Identification of the key gene related to Lauren classification. A - L Overall survival and disease-free survival curves for six key genes in the TCGA and GSE62254 databases (MFAP4, MYL9, MYH11, LMOD1, FXYD6, VIP). M Relationship between gene expression of LMOD1 and Lauren classification of patients. N The correlation between LMOD1 and HER-2 (ERBB2) expression in TCGA and GEO databases. M Analysis with the MCOD algorithm yielding related sub-modules of LMOD1 in blue cyan

Article Snippet: The primary antibodies used were: LMOD1 (Accept), FAK (Proteintech), AKT (CST), p-AKT (CST), mTOR (Proteintech), p-mTOR (Proteintech), E-cadherin (CST), N-cadherin (CST), vimentin (CST), and actin (Proteintech).

Techniques: Gene Expression, Expressing

Journal: BMC Cancer

Article Title: LMOD1, an oncogene associated with Lauren classification, regulates the metastasis of gastric cancer cells through the FAK-AKT/mTOR pathway

doi: 10.1186/s12885-022-09541-0

Figure Lengend Snippet: LMOD1 enhanced the migration ability of gastric cancer cells through the FAK-Akt/mTOR pathway. A The expression of LMOD1 in cell lines was measured using western blots. B The expression of LMOD1 in cell lines was measured by RT-qPCR. C - F A GSEA analysis of LMOD1 enriched pathways in TCGA and GSE62254 databases. G , H Western blotting was used to measure protein markers of the FAK-Akt/mTOR pathway in Control or LMOD1- silenced SGC-7901 and MGC-803 cell lines. I Western blotting was used to measure protein markers of the FAK-Akt/mTOR pathway in the Control or LMOD1-overexpressed HGC-27 cell line. J , K The migration ability of cells was detected using a scratch test. M , N The migration ability of cells was measured using a transwell test. The membrane was cut out due to the different secondary antibodies incubated. *, P < 0.05, **, P < 0.01, ***, P < 0.001, ****, P < 0.0001

Article Snippet: The primary antibodies used were: LMOD1 (Accept), FAK (Proteintech), AKT (CST), p-AKT (CST), mTOR (Proteintech), p-mTOR (Proteintech), E-cadherin (CST), N-cadherin (CST), vimentin (CST), and actin (Proteintech).

Techniques: Migration, Expressing, Western Blot, Quantitative RT-PCR, Control, Membrane, Incubation

Journal: BMC Cancer

Article Title: LMOD1, an oncogene associated with Lauren classification, regulates the metastasis of gastric cancer cells through the FAK-AKT/mTOR pathway

doi: 10.1186/s12885-022-09541-0

Figure Lengend Snippet: LMOD1 promoted peritoneal metastasis of gastric cancer cells by regulating the epithelial-mesenchymal transition. A , B GSEA analysis of LMOD1 enriched pathways in TCGA and GSE62254 databases. C , D Western blotting was used to verify the regulation of epithelial-mesenchymal transition (EMT)-related proteins in Control or shLMOD1 SGC-7901 and MGC-803 cell lines. E Western blotting was used to verify the regulation of EMT-related proteins in Control/\ or overexpressed-LMOD1 HGC-27 cell lines. F - H The adhesion ability of cells was verified using an adhesion test. I - K An invasion assay was used to verify the ability of peritoneal infiltration. L , M The effect of LMOD1 silencing on invasion and migration of gastric cancer cells in SGC-7901 cells was verified by intraperitoneal metastasis model in nude mice. We cut the membrane to facilitate the better hybridization of antibodies and avoid the interaction of antibodies. *, P < 0.05, **, P < 0.01, ***, P < 0.001, ****, P < 0.0001

Article Snippet: The primary antibodies used were: LMOD1 (Accept), FAK (Proteintech), AKT (CST), p-AKT (CST), mTOR (Proteintech), p-mTOR (Proteintech), E-cadherin (CST), N-cadherin (CST), vimentin (CST), and actin (Proteintech).

Techniques: Western Blot, Control, Invasion Assay, Migration, Membrane, Hybridization

Journal: BMC Cancer

Article Title: LMOD1, an oncogene associated with Lauren classification, regulates the metastasis of gastric cancer cells through the FAK-AKT/mTOR pathway

doi: 10.1186/s12885-022-09541-0

Figure Lengend Snippet: Expression and survival significance of LMOD1 in clinical tissue samples. A , B Western blotting was used to measure the protein expression of LMOD1 in 48 pairs of gastric cancer tissues. C , D LMOD1 protein expression was measured by immunohistochemistry in 102 cases of gastric cancer tissues and 40 cases of adjacent tissues. E Overall survival curve of LMOD1in gastric cancer. F Overall survival curve of LMOD1 in diffuse gastric cancer. G Schematic diagram showed that the regulatory effect of LMOD1 on peritoneal metastasis in diffused gastric cancer. The membrane was cut out due to the different secondary antibodies incubated. ****, P < 0.0001

Article Snippet: The primary antibodies used were: LMOD1 (Accept), FAK (Proteintech), AKT (CST), p-AKT (CST), mTOR (Proteintech), p-mTOR (Proteintech), E-cadherin (CST), N-cadherin (CST), vimentin (CST), and actin (Proteintech).

Techniques: Expressing, Western Blot, Immunohistochemistry, Membrane, Incubation

Journal: BMC Cancer

Article Title: LMOD1, an oncogene associated with Lauren classification, regulates the metastasis of gastric cancer cells through the FAK-AKT/mTOR pathway

doi: 10.1186/s12885-022-09541-0

Figure Lengend Snippet: Correlation between LMOD1 expression and clinicopathologic characteristics in GC patients

Article Snippet: The primary antibodies used were: LMOD1 (Accept), FAK (Proteintech), AKT (CST), p-AKT (CST), mTOR (Proteintech), p-mTOR (Proteintech), E-cadherin (CST), N-cadherin (CST), vimentin (CST), and actin (Proteintech).

Techniques: Expressing